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In 1982, during her sophomore year of nursing school, she started working as a stripper at Mitchell Brothers O'Farrell Theater.
She made her foray into the world of pornographic movies during her junior year, in 1984. Her debut performance was in the film Educating Nina, which was produced by the veteran porn star Juliet Anderson (better known as "Aunt Peg"), and this proved to be a massive hit. Since then she has gone on to feature in over 400 first-run adult films, and has become one of the most enduring and recognizable performers in the industry.
Hartley does not have any children due to a combination of a lack of interest and fibroid tumors, though she does have eight nieces and nephews. On October 3rd 2008, Hustler announced that Hartley would play the part of Hillary Rodham Clinton in their upcoming film Who's Nailin' Paylin?, a pornographic parody of vice-presidential candidate Sarah Palin with Lisa Ann in the role of Palin.