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Sprinkle is known as the "prostitute and porn star turned sex educator and artist". Her best known theater and performance art piece is her Public Cervix Announcement, in which she invites the audience to "celebrate the female body" by viewing her cervix with a speculum and flashlight. She also performed The Legend of the Ancient Sacred Prostitute, in which she did a "sex magic" masturbation ritual on stage. She has toured one-woman shows internationally for 17 years, some of which were are titled Post Porn Modernist, Annie Sprinkle's Herstory of Porn, Hardcore from the Heart, and, currently, Exposed; Experiments in Love, Sex, Death and Art.
The first porn star known to have earned a Ph.D., Sprinkle received her Ph.D. in Human Sexuality from the Institute for Advanced Study of Human Sexuality in San Francisco. Her work, spanning more than three decades, is studied at many universities, in theater history, women's studies, and film studies courses. She also is a faculty member at The New School of Erotic Touch.